The Chemical Biology of Nucleic Acids

This book focuses on the methods for obtaining modified and native nucleic acids, and their biological applications.

Topics covered include:

* chemical synthesis of modified RNA

* expansion of the genetic alphabet in nucleic acids by creating new base pairs

* chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides

* nucleic-acid-templated chemistry

* chemical biology of peptide nucleic acids (PNA)

* the interactions of small molecules with DNA and RNA

* the architectural modules of folded RNAs

* genesis and biological applications of locked nucleic acid (LNA)

* small non-coding RNA in bacteria

* microRNA-guided gene silencing

* nucleic acids based therapies

* innate immune recognition of nucleic acid

* light-responsive nucleic acids for the spatiotemporal control of biological processes

* DNA methylation

* frameworks for programming RNA devices

* RNA as a catalyst: The Diels-Alderase-Ribozyme

* evolving an understanding of RNA function by in vitro approaches

* the chemical biology of aptamers: synthesis and applications

* nucleic acids as detection tools

* bacterial riboswitch discovery and analysis