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E. coli Gene Expression Protocols
Description
Gene
expression using E. coli as a host is carried out over a wide range
of disciplines in academic and industrial laboratories. In E. coli
Gene Expression Protocols, Peter E. Vaillancourt presents a
collection of popular and emerging methodologies that take advantage
of E. coli's ability to quickly and inexpensively express recombinant
proteins. The authors focus on two areas of interest: the use of E.
coli vectors and strains for production of pure, functional protein,
and the use of E. coli as host for the functional screening of large
collections of proteins and peptides. Among the cutting-edge
techniques demonstrated are those for rapid high-level expression and
purification of soluble and functional recombinant protein, and those
essential to functional genomics, proteomics, and protein
engineering. Described in step-by-step detail to ensure robust,
trouble-free results, each proven method has been written by a
hands-on expert and includes extensive notes and practical tips for
avoiding pitfalls. Even highly skilled researchers will find many
time-saving techniques.
Authoritative and highly practical,
E. coli Gene Expression Protocols provides a state-of-the-art
collection of tested methods for this powerful gene expression
technology, offering today's investigators proven tools for success
in the emerging fields of functional genomics and proteomics.
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